Enzyme Action

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In this activity that can be used as a lab or demonstration, learners use Lactaid® and lactose to demonstrate the concept of enzyme action. Learners test a drop of milk and Lactaid® for the presence of glucose using glucose test paper. Learners also discover the color range of glucose test paper readings. In addition, learners construct paper models to help visualize enzyme action.

Quick Guide

Preparation Time:
Under 5 minutes

Learning Time:
10 to 30 minutes

Estimated Materials Cost:
Over $20 per group of students

Age Range:
Ages 14 - 18

Resource Types:
Activity, Demonstration, Experiment/Lab Activity, Lesson/Lesson Plan


Materials List (per group of students)

  • Spot plate or glass slide
  • Marker
  • Toothpick for mixing
  • Forceps for handling the glucose test paper
  • Milk-whole, skim or 1%
  • Droppers
  • Lactaid® (lactase source), the liquid form works best
  • Glucose test strips
  • Any glucose solution
  • Any sucrose solution
  • Paper models of enzyme, HOH, galactose, glucose, lactose


  • Life Sciences
    • Cells
    • Human Body
      • Digestion
      • Health and Nutrition
  • Physical Sciences
    • Chemistry
      • Chemical Reactions
    • Structure and Properties of Matter
  • The Nature of Science
    • The Scientific Process
      • Conducting Investigations
      • Gathering Data
      • Formulating Explanations
      • Communicating Results

Informal Categories

  • Food and Cooking


To use this activity, learners need to:

  • see
  • see color
  • read
  • touch

Learning styles supported:

  • Involves hands-on or lab activities


This resource is part of:

Access Rights:

  • Free access


  • Crumlish, Jane