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In this investigation learners explore the differences between, and interdependence of, living and nonliving elements in a water ecosystem.

In this activity, learners create a freshwater ecosystem in a large plastic bottle. Learners cut and prepare bottles, then fill with water, aquatic plants, snails and fish.

In this activity (on pages 13-16 of the PDF) learners investigate three mystery samples to see which one contains life. The three samples are sand, sand and yeast, and sand and antacid.